Buchegger A, Nemeth P M, Pette D, Reichmann H
J Physiol. 1984 May;350:109-19. doi: 10.1113/jphysiol.1984.sp015191.
Chronic indirect stimulation (10 Hz) was performed on rabbit tibialis anterior muscle. Long-term stimulation (52-140 days) produced a transformation of the fast tibialis anterior into a slow red muscle as judged from the histochemistry of myofibrillar actomyosin ATPase, the pattern of myosin light chains and the thorough rearrangement of the enzyme activity pattern of energy metabolism. Activity levels of citrate synthetase (CS), malate dehydrogenase (MDH), succinate dehydrogenase (SDH), 3-hydroxy-acyl-CoA dehydrogenase (HAD), and lactate dehydrogenase (LDH) were determined quantitatively by either microbiochemical assays (CS, MDH, HAD and LDH) on microdissected, single fibres or by kinetic microphotometry on cross-sectioned fibres (SDH). The activity profiles of these enzymes displayed pronounced scattering in the fibre population of the unstimulated muscle. Despite a several fold increase in the activities of CS, MDH, SDH and HAD and a pronounced decrease in LDH, chronic stimulation failed to abolish the metabolic heterogeneity of the fibre population. It is possible that chronic indirect stimulation cannot produce uniformity of fibres because of continuing diverse natural activity of the motor units.
对兔胫前肌进行慢性间接刺激（10赫兹）。长期刺激（52 - 140天）使快速型胫前肌转变为慢速红色肌肉，这是根据肌原纤维肌动球蛋白ATP酶的组织化学、肌球蛋白轻链模式以及能量代谢酶活性模式的彻底重排判断得出的。通过对显微解剖的单根纤维进行微生物化学分析（柠檬酸合酶、苹果酸脱氢酶、3 - 羟基 - 酰基辅酶A脱氢酶和乳酸脱氢酶）或对横切纤维进行动力学显微光度测定（琥珀酸脱氢酶），定量测定柠檬酸合酶（CS）、苹果酸脱氢酶（MDH）、琥珀酸脱氢酶（SDH）、3 - 羟基 - 酰基辅酶A脱氢酶（HAD）和乳酸脱氢酶（LDH）的活性水平。这些酶的活性谱在未受刺激肌肉的纤维群体中表现出明显的离散。尽管CS、MDH、SDH和HAD的活性增加了几倍，LDH明显降低，但慢性刺激未能消除纤维群体的代谢异质性。由于运动单位持续存在不同的自然活动，慢性间接刺激可能无法使纤维产生均匀性。
