Liu P I, Sarji N M, Poon K, Liu S S
Ann Clin Lab Sci. 1982 Jan-Feb;12(1):11-5.
Acid phosphatase levels in pure lymphocytes from normal individuals were determined cytochemically on intact cells and spectrophotometrically on cell extracts. Good correlation was demonstrated between the results obtained with both methods. Biochemical assay showed that a normal range of 7.2 +/- 0.9 and 7.1 +/- 1.0 mMoles per 10(6) lymphocytes was obtained without and with tartrate inhibition, respectively. A significant loss of acid phosphatase level in the extract was found on storage. Seventy-five, 49 and 45 percent of the acid phosphatase activity remained after 3.5, 24, and 48 hours refrigeration at 4 degrees C, with 51 percent and 32 percent after 24 and 48 hours refrigeration at -20 degrees C, respectively. The spectrophotometric assay is less variable than the cytochemical stain. The widely utilized cytochemical method, although less reproducible, allows for evaluation of intracellular distribution of the enzyme in addition to level of activity.
采用细胞化学方法对正常个体的纯淋巴细胞完整细胞进行酸性磷酸酶水平测定，并采用分光光度法对细胞提取物进行测定。两种方法所得结果之间显示出良好的相关性。生化分析表明，在不添加酒石酸盐抑制和添加酒石酸盐抑制的情况下，每10⁶个淋巴细胞的酸性磷酸酶正常范围分别为7.2±0.9和7.1±1.0毫摩尔。提取物中的酸性磷酸酶水平在储存时显著降低。在4℃冷藏3.5、24和48小时后，分别有75%、49%和45%的酸性磷酸酶活性保留，在-20℃冷藏24和48小时后，分别有51%和32%的活性保留。分光光度法测定的变异性小于细胞化学染色。广泛使用的细胞化学方法虽然重复性较差，但除了能评估酶活性水平外，还能评估酶在细胞内的分布情况。
