Botstein G R, Sherer G K, Leroy E C
Arthritis Rheum. 1982 Feb;25(2):189-95. doi: 10.1002/art.1780250212.
Human preputial skin fibroblasts were cloned in vitro, and studies in 21 clones were shown to exhibit a 3-fold difference in collagen synthesis; this heterogeneity of phenotypic expression was conserved over multiple population doublings. When the clones were exposed to sera from 10 scleroderma patients, selective growth of high-collagen-producing fibroblasts was observed. Prostaglandin E2 abrogated the selective effect of scleroderma serum on high-collagen-producing fibroblast clones. Besides enhanced collagen synthesis, these clones of normal fibroblasts shared an insensitivity to feeding schedule with fibroblasts from scleroderma skin. These data raise the possibility that scleroderma represents a process of selection of fibroblasts programmed to produce increased amounts of collagen.
人包皮皮肤成纤维细胞在体外进行克隆，对21个克隆的研究表明，它们在胶原蛋白合成方面存在3倍的差异；这种表型表达的异质性在多次群体倍增过程中得以保留。当这些克隆暴露于10例硬皮病患者的血清中时，观察到高胶原蛋白产生的成纤维细胞出现选择性生长。前列腺素E2消除了硬皮病血清对高胶原蛋白产生的成纤维细胞克隆的选择性作用。除了胶原蛋白合成增强外，这些正常成纤维细胞克隆与硬皮病皮肤来源的成纤维细胞一样，对培养时间表不敏感。这些数据增加了硬皮病代表一个选择程序设定为产生更多胶原蛋白的成纤维细胞的过程的可能性。
