Champion B R, Poole A R
Arthritis Rheum. 1982 Mar;25(3):274-87. doi: 10.1002/art.1780250305.
Cellular immunity to rabbit types I, II, and III collagen was detected after both partial medial meniscectomy and sham operation in rabbits when an in vitro 3H-thymidine incorporation assay was used. The blastogenic responses were commonly directed towards peptides derived from collagens by cyanogen bromide cleavage used to mimic proteinase degradation products. The responses to type III collagen peptides were by far the strongest and were seen in most rabbits that were operated on. We suggest that the responses to types I and II collagen were caused by a cross-reaction with type III collagen peptides. This conclusion was supported by the observation that spleen cells from rabbits directly immunized with homologous type III collagen peptides in Freund's complete adjuvant responded strongly to the immunizing peptides and also cross-reacted with types I and II collagen peptides. Immunity to cartilage proteoglycans was observed primarily in rabbits that had undergone meniscectomy and had severe cartilage degeneration. These results indicated that immunity to collagens will develop merely as a result of joint surgery, whereas immunity to proteoglycans is largely dependent upon an osteoarthritic lesion.
当采用体外3H-胸腺嘧啶核苷掺入试验时，在兔内侧半月板部分切除术和假手术后均检测到对兔I型、II型和III型胶原的细胞免疫。增殖反应通常针对通过溴化氰裂解胶原衍生的肽，以模拟蛋白酶降解产物。对III型胶原肽的反应迄今为止最为强烈，在大多数接受手术的兔中都可见到。我们认为，对I型和II型胶原的反应是由与III型胶原肽的交叉反应引起的。这一结论得到以下观察结果的支持：在弗氏完全佐剂中用同源III型胶原肽直接免疫的兔的脾细胞，对免疫肽有强烈反应，并且也与I型和II型胶原肽发生交叉反应。对软骨蛋白聚糖的免疫主要在接受半月板切除术且有严重软骨退变的兔中观察到。这些结果表明，仅关节手术就会产生对胶原的免疫，而对蛋白聚糖的免疫很大程度上取决于骨关节炎病变。
