Cockle S M, Dean R T
Biosci Rep. 1982 Feb;2(2):107-14. doi: 10.1007/BF01116176.
Changes in endogenous protein degradation of stable proteins with growth state have been observed in a number of normal cell lines: increases in degradation of between 15 and 40% occurred in 10 cell lines at confluence. No such regulation of proteolysis was observed in 4 cell lines which were clonogenic in soft agar. We have evidence that this derangement of regulation coincides with the transformation of cells, because such regulation disappears in spontaneously transforming fibroblasts and is decreased by the tumour promoter 12-0-tetradecanoyl-phorbol-13-acetate. In addition, regulation reappears in a growth control revertant of a transformed line.
10个细胞系在汇合时蛋白质降解增加了15%至40%。在4个能在软琼脂中克隆生长的细胞系中未观察到这种蛋白水解调节。我们有证据表明这种调节紊乱与细胞转化同时发生，因为这种调节在自发转化的成纤维细胞中消失，并且会被肿瘤启动子12-0-十四烷酰佛波醇-13-乙酸酯降低。此外，在一个转化细胞系的生长控制回复突变体中调节作用又重新出现。
