Robin J, Dery C
Can J Microbiol. 1982 Jan;28(1):58-64. doi: 10.1139/m82-003.
The EFDL strain of Bluegill virus , fish virus, was grown in a Bluegill Fry cell line (BF-2) in medium containing [3H] uridine or [32P] orthophosphate. This virus was purified from the cellular material by precipitation with PEG 6000 followed by isopycnic centrifugation in a Percoll gradient. As calculated from reconstruction experiments, only 8.5 and 0.5%, respectively, of the host proteins and the host RNA were recovered with the viral band, whereas the recovery of viral infectivity was approximately 20%. Electron microscopy of the viral band showed mostly intact virus particles. The nucleic acid extracted from purified virus was found to have the following properties: (i) it migrated homogeneously during electrophoresis at the rate expected from a single-stranded nucleic acid of molecular weight 2.17 X 10(6), (ii) it sedimented as a single molecular species when analyzed by velocity centrifugation in sucrose gradients, (iii) it banded in a neutral Cs2SO4 gradient at a density of 1.69 g/cm3 as expected for single-stranded RNA, (iv) its base composition is that of a single-stranded RNA molecule: 14.89% C, 12.77% A, 37.03% U, and 35.23% G. The nucleic acid of Bluegill virus thus appears to be a single-stranded RNA molecule of approximately 2.17 X 10(6) molecular weight.
蓝鳃太阳鱼病毒的EFDL毒株是一种鱼类病毒，在含有[3H]尿苷或[32P]正磷酸盐的培养基中的蓝鳃太阳鱼幼体细胞系（BF-2）中培养。该病毒通过用PEG 6000沉淀，随后在Percoll梯度中进行等密度离心，从细胞物质中纯化出来。根据重建实验计算，病毒带中仅分别回收了8.5%和0.5%的宿主蛋白和宿主RNA，而病毒感染性的回收率约为20%。病毒带的电子显微镜检查显示大多为完整的病毒颗粒。从纯化病毒中提取的核酸具有以下特性：（i）在电泳过程中以分子量为2.17×10(6)的单链核酸预期的速率均匀迁移；（ii）在蔗糖梯度中通过速度离心分析时作为单一分子种类沉降；（iii）在中性Cs2SO4梯度中以1.69 g/cm3的密度形成条带，这是单链RNA预期的密度；（iv）其碱基组成是单链RNA分子的碱基组成：14.89% C、12.77% A、37.03% U和35.23% G。因此，蓝鳃太阳鱼病毒的核酸似乎是分子量约为2.17×10(6)的单链RNA分子。
