Kamura T, Nielsen O S, Overgaard J, Andersen A H
Cancer Res. 1982 May;42(5):1744-8.
The effect of 43.5 degrees water bath heating on a C3H mammary carcinoma inoculated into the foot of BALB/c x DBA F1 (hereafter called CD2F1 mice was investigated. A single heat treatment resulted in a linear dose-response relationship between heating time and tumor growth time (i.e., the time for tumors to reach 5 times the initial volume of the first treatment day). Recovery from hyperthermic damage, demonstrated by two-dose fractionation experiments (30 min + 60 min at 43.5 degrees), increased with increasing fractionation interval and reached its maximum at a 16-hr interval. Preheating for 30 min at 43.5 degrees induced thermal resistance to a second heat treatment at 43.5 degrees (thermotolerance) which was evidenced by a decrease in the slope of the dose-response curves. This thermotolerance gradually increased with increasing interval and reached a maximum at a 16-hr interval with a thermotolerance ratio of 5.2. Subsequently, the thermotolerance gradually decayed and completely disappeared at a 120-hr interval. No detectable repair of hyperthermic damage was found in this tumor. In principle, there data confirm the observations on thermotolerance reported previously for cell cultures in vitro and for several normal tissues in vivo.
研究了43.5℃水浴加热对接种于BALB/c×DBA F1（以下简称CD2F1小鼠）足部的C3H乳腺癌的影响。单次热处理导致加热时间与肿瘤生长时间（即肿瘤体积达到首次治疗日初始体积5倍所需的时间）之间呈线性剂量反应关系。通过两剂量分割实验（43.5℃下30分钟+60分钟）证明，热损伤的恢复随分割间隔的增加而增加，并在16小时间隔时达到最大值。在43.5℃下预热30分钟可诱导对43.5℃第二次热处理的热抗性（热耐受），这可通过剂量反应曲线斜率的降低来证明。这种热耐受随间隔时间的增加而逐渐增加，并在16小时间隔时达到最大值，热耐受比为5.2。随后，热耐受逐渐衰减，并在120小时间隔时完全消失。在这种肿瘤中未发现热损伤的可检测修复。原则上，这些数据证实了先前关于体外细胞培养和体内几种正常组织热耐受的观察结果。
