Nielsen O S, Overgaard J
Br J Cancer. 1982 Dec;46(6):894-903. doi: 10.1038/bjc.1982.299.
The importance of the priming heat treatment temperature and heating time for the degree and kinetics of thermotolerance was investigated in a C3H mammary carcinoma inoculated into the feet of CDF1 mice. A single heat treatment in the range 41.5-44.5 degrees C resulted in a linear relationship between heating time and tumour growth time (i.e. the time for tumours to reach a volume five times that of the first treatment day). An Arrhenius plot showed an inflection point at 42.5 degrees C with activation energies of 635 and 1508 kJ/mol, respectively, above and below 42.5 degrees C. The degree and kinetics of thermotolerance were independent of the preheating temperature, if the heating time was adjusted to give the same level of heat damage. A pretreatment at these temperatures with a tumour growth time of approximately 10 days, equivalent to 30 min at 43.5 degrees C, resulted in maximal thermotolerance at a 16-h interval with a thermotolerance ratio (TTRmax) of approximately 5.2. Preheating of the tumours at 43.5 degrees C for 3.5, 7.5, 15, 30, or 45 min, showed that if the preheating time was increased, both the TTRmax and the time interval necessary to develop TTRmax increased, both being linear functions of the duration of the preheating time. Maximal thermotolerance was obtained at intervals of 2, 4, 8, 16, and 28 h with TTRmax of 1.6, 2.2, 3.7, 5.2, and 7.7, respectively.
将C3H乳腺癌接种到CDF1小鼠足部，研究引发热处理温度和加热时间对热耐受性程度及动力学的影响。41.5 - 44.5摄氏度范围内的单次热处理导致加热时间与肿瘤生长时间（即肿瘤体积达到首次治疗日体积五倍所需的时间）呈线性关系。阿累尼乌斯图显示在42.5摄氏度处有一个拐点，42.5摄氏度以上和以下的活化能分别为635和1508 kJ/mol。如果调整加热时间以产生相同程度的热损伤，热耐受性的程度和动力学与预热温度无关。在这些温度下进行预处理，肿瘤生长时间约为10天，相当于43.5摄氏度下30分钟，在16小时间隔时产生最大热耐受性，热耐受性比率（TTRmax）约为5.2。在43.5摄氏度下将肿瘤预热3.5、7.5、15、30或45分钟，结果表明，如果预热时间增加，TTRmax和产生TTRmax所需的时间间隔都会增加，二者均为预热时间的线性函数。在2、4、8、16和28小时的间隔时获得最大热耐受性，TTRmax分别为1.6、2.2、3.7、5.2和7.7。
