Smith R J, Koenig R J, Binnerts A, Soeldner J S, Aoki T T
J Clin Invest. 1982 May;69(5):1164-8. doi: 10.1172/jci110552.
The formation of hemoglobin AIc was studied in intact human erythrocytes in vitro. Satisfactory methods were developed for maintaining erythrocytes under physiologic conditions for greater than 8 d with less than 10% hemolysis. Hemoglobin AIc levels were determined chromatographically on erythrocyte hemolysates after removal of reversible components by incubation for 6 h at 37 degree C. Hemoglobin AIc concentration was found to increase linearly with time during 8 d of incubation. The rate of formation of hemoglobin AIc increased linearly as glucose concentration was increased from 40 to 1,000 mg/dl. Deoxyhemoglobin was glycosylated twice as rapidly as oxyhemoglobin. The rate of hemoglobin AIc formation was further increased by elevated 2,3-diphosphoglycerate levels, an effect that was most marked with deoxyhemoglobin. We conclude that the nonenzymatic glycosylation of hemoglobin is influenced by factors other than glucose, including oxygen tension and 2,3-diphosphoglycerate levels.
在体外对完整的人红细胞中糖化血红蛋白AIc的形成进行了研究。开发出了令人满意的方法，可在生理条件下将红细胞维持8天以上，溶血率低于10%。在37℃孵育6小时以去除可逆成分后，通过色谱法测定红细胞裂解液中的糖化血红蛋白AIc水平。发现在孵育8天期间，糖化血红蛋白AIc浓度随时间呈线性增加。随着葡萄糖浓度从40增加到1000mg/dl，糖化血红蛋白AIc的形成速率呈线性增加。脱氧血红蛋白的糖基化速度是氧合血红蛋白的两倍。2,3-二磷酸甘油酸水平升高进一步提高了糖化血红蛋白AIc的形成速率，这种效应在脱氧血红蛋白中最为明显。我们得出结论，血红蛋白的非酶糖基化受葡萄糖以外的因素影响，包括氧张力和2,3-二磷酸甘油酸水平。
