Donaldson K, Cullen R T
Br J Exp Pathol. 1984 Feb;65(1):81-90.
Chemiluminescence, a measure of reactive oxygen release by phagocytes, was compared in peritoneal exudate macrophages elicited with chrysotile asbestos, Corynebacterium parvum and saline. Chrysotile asbestos- and C. parvum-activated macrophages produced significantly more chemiluminescence than saline-elicited macrophages. In a second series of experiments the ability of opsonized chrysotile asbestos to act as a trigger for the release of chemiluminescence was tested. Opsonized chrysotile asbestos produced a dose-related release of chemiluminescence from activated macrophages except at the highest dose where chemiluminescence was reduced due, possibly, to a toxic effect of chrysotile during the assay. Opsonized latex also triggered a dose-related chemiluminescent response from activated macrophages. The potential role of toxic reactive oxygen species, released from macrophages, in the development of asbestos-related pulmonary inflammation and fibrosis are discussed.
比较了由温石棉、短小棒状杆菌和生理盐水引发的腹膜渗出巨噬细胞中化学发光（一种衡量吞噬细胞释放活性氧的指标）。温石棉和短小棒状杆菌激活的巨噬细胞产生的化学发光明显多于生理盐水引发的巨噬细胞。在第二系列实验中，测试了调理过的温石棉作为化学发光释放触发因素的能力。调理过的温石棉从激活的巨噬细胞中产生了与剂量相关的化学发光释放，但在最高剂量时除外，此时化学发光可能因温石棉在检测过程中的毒性作用而降低。调理过的乳胶也引发了激活巨噬细胞的剂量相关化学发光反应。讨论了巨噬细胞释放的有毒活性氧在石棉相关肺部炎症和纤维化发展中的潜在作用。
