Zhuravkin I N, Kozlov V A
Tsitologiia. 1982 Feb;24(2):200-5.
Polypotential hemopoietic stem cell numbers were determined by means of the splenic colony-forming unit (CFU-S) method in bone marrow and spleen of CBA mice in different periods after bleeding and acute hypoxia. After these treatments, radioactive isotope (59Fe) uptake by erythroid cells, considered as an index of erythropoietic activity, enhanced in the spleen, but in the bone marrow it either diminished (after acute hypoxia), or remained unchanged (after bleeding). Bleeding caused a considerable increase in the splenic CFU-S numbers, but the bone marrow CFU-S contents were unchanged. It was found that splenic CFU-S number decreased on the 1st and 4th days after acute hypoxia, but increased on the 7th day; bone marrow CFU-s numbers increased on the 7th day. Possible mechanisms involved in the hemopoietic stem cell perturbation in the hemopoietic organs after the erythropoiesis stimulating treatment are discussed.
采用脾集落形成单位（CFU-S）法，测定不同时期放血和急性缺氧后CBA小鼠骨髓和脾脏中多能造血干细胞数量。这些处理后，作为红细胞生成活性指标的红细胞对放射性同位素（59Fe）的摄取在脾脏中增强，但在骨髓中，要么减少（急性缺氧后），要么保持不变（放血后）。放血导致脾脏CFU-S数量显著增加，但骨髓CFU-S含量未变。结果发现，急性缺氧后第1天和第4天脾脏CFU-S数量减少，但第7天增加；骨髓CFU-S数量在第7天增加。讨论了促红细胞生成治疗后造血器官中造血干细胞扰动的可能机制。
