Hughes A, Mijović V, Brozović B, Davies T D
Vox Sang. 1982 Mar;42(3):145-50. doi: 10.1111/j.1423-0410.1982.tb01085.x.
Non-haemolytic febrile transfusion reactions are common in multi-transfused patients. It is generally accepted that the majority of these reactions occur when more than 0.5 x 10(9) leucocytes are transfused. Values equal to or lower than this threshold, which we have called for convenience the critical antigenic load for leucocytes (CALL), can be achieved by decreasing the leucocyte content in one unit of whole stored blood by about 80%. We have compared the efficiency of leucocyte depletion of whole stored blood, using fixed-speed centrifugation (Haemonetics Model 15 and Model 115 cell washer), variable-speed centrifugation (Dideco Progress 90 Cell Separator), and serial centrifugation (IBM 2991 Blood Cell Processor. Fixed-speed centrifugation was least effective in depletion leucocytes; a reduction of 25 and 60% was achieved using the Haemonetics Model 15 and Model 115, respectively. Variable speed and serial centrifugation produced more satisfactory results with leucocyte depletions of 82 and 89% using the Dideco Progress 90 Cell Separator and the IBM 2991 Blood Cell Processor, respectively. Platelet depletion of over 90% was achieved with all the cell washers, except the Haemonetics Model 15. Red cell losses varied from 3 to 30%. It seems unlikely that consistently high (over 90%) leucocyte depletion can be obtained using cell-washing techniques without associated high red cell losses.
非溶血性发热性输血反应在多次输血的患者中很常见。一般认为，当输入超过0.5×10⁹个白细胞时，这些反应大多会发生。我们为方便起见将此阈值称为白细胞的临界抗原负荷（CALL），通过将一单位全血储存液中的白细胞含量降低约80%，可达到等于或低于该阈值的值。我们比较了使用定速离心（Haemonetics 15型和115型细胞洗涤器）、变速离心（Dideco Progress 90细胞分离器）和连续离心（IBM 2991血细胞处理器）对全血储存液进行白细胞去除的效率。定速离心在去除白细胞方面效果最差；使用Haemonetics 15型和115型分别可使白细胞减少25%和60%。变速离心和连续离心产生了更令人满意的结果，使用Dideco Progress 90细胞分离器和IBM 2991血细胞处理器分别使白细胞减少82%和89%。除了Haemonetics 15型外，所有细胞洗涤器的血小板去除率均超过90%。红细胞损失率在3%至30%之间。使用细胞洗涤技术似乎不太可能在不伴有高红细胞损失的情况下持续获得高（超过90%）的白细胞去除率。
