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[3H]胸腺嘧啶核苷掺入海胆强壮柱头虫基因组中具有不同重复核苷酸序列频率的DNA组分中]

[3H]Thymidine incorporation into DNA fractions with differing frequencies of repetitive nucleotide sequences in the sea urchin strongylocentrotus droebachiensis genome].

作者信息

Andreeva T F, Andreeva L F

出版信息

Biokhimiia. 1982 Mar;47(3):385-9.

PMID:7074168
Abstract

The dynamics of [3H]thymidine incorporation into DNA fractions with highly repetitive, moderately repetitive and unique nucleotide sequences in the genome of sea urchin embryos at the cleavage stage (8-32 blastomers) were studied. The ratio of specific radioactivity of the DNA fractions to the specific radioactivity of the original DNA showed that for each of the points of incubation with [3H]thymidine the relative specific radioactivities of different DNA fractions differed from each other. The highly repetitive DNA fraction has a very high relative specific radioactivity at the beginning of incubation (270% in 10 min), but in 40 min its the relative specific radioactivities of the DNA fractions with moderately repetitive and unique nucleotide sequences are not high at the beginning of incubation (40 and 75%, respectively) and then gradually increase up to 80 and 120%, respectively.

摘要

研究了在海胆胚胎卵裂期(8 - 32个卵裂球)基因组中,[3H]胸苷掺入具有高度重复、中度重复和单拷贝核苷酸序列的DNA组分的动力学。DNA组分的比放射性与原始DNA的比放射性之比表明,在与[3H]胸苷孵育的每个时间点,不同DNA组分的相对比放射性彼此不同。高度重复DNA组分在孵育开始时具有非常高的相对比放射性(10分钟内为270%),但在40分钟时,中度重复和单拷贝核苷酸序列的DNA组分的相对比放射性在孵育开始时不高(分别为40%和75%),然后逐渐分别增加到80%和120%。

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