[中间球海胆DNA中回文序列的特征]
[Characteristics of palindromic sequences in DNA of the sea urchin Strongylocentrotus intermedius].
作者信息
Brykov V A, Kukhlevskiĭ A D
出版信息
Biokhimiia. 1985 Sep;50(9):1553-9.
Some properties of the palindromic sequences in the sea urchin Strongylocentrotus intermedius nuclear DNA have been studied. It was shown that the amount of "foldback HAP bound DNA" and the S1 nuclease resistant DNA depends on renaturation temperature and Na+ concentration in solution. The authentic fraction of inverted repeats comprises 10-15% of the total DNA. The complexity of the palindromic fraction is approximately 8,2 X 10(7) nucleotide pairs and the average number of inverted repeats approximates 5 X 10(5) per haploid genome. The renaturation kinetics of inverted repeats with excess of total homologous DNA indicates that these sequences are enriched with unique DNA. The possible function of palindromic sequences is discussed.
对中间球海胆核DNA中回文序列的一些特性进行了研究。结果表明，“折回HAP结合DNA”和S1核酸酶抗性DNA的量取决于复性温度和溶液中的Na+浓度。反向重复序列的真实比例占总DNA的10-15%。回文部分的复杂度约为8.2×10⁷个核苷酸对，每个单倍体基因组中反向重复序列的平均数量约为5×10⁵个。反向重复序列与过量的总同源DNA的复性动力学表明，这些序列富含独特DNA。文中讨论了回文序列可能的功能。
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