Merzliak M N, Pogosian S I, Rumiantseva V B, Sobolev A S, Shevchenko N V
Biokhimiia. 1982 Mar;47(3):425-33.
The chromatographic properties (e. g. thin layer mobility, separation by molecular adsorption and gel chromatography) and the fluorescence parameters of liposoluble fluorescent compounds (LFC) accumulated in the tissues during aging of detached leaves, in autumn leaves of deciduous plants and in the leaves treated with diquat, 2,4-dichloro- and 2,4,5-trichlorophenoxyacetic acids were studied. The peculiarities of detection and analysis of LFC in plant tissues are discussed. Fractionation of individual components of LFC was carried out and their properties were compared. The properties of LFC were compared to those of lipofuscine-like animal pigments and fluorescent products formed by NADPH-dependent lipid peroxidation system in rat liver microsomes stimulated by reduced pyridine nucleotides in pea leaf microsomes and by lipid photoperoxidation in isolated chloroplasts. The data obtained suggest that accumulation of "heavy" LFC fractions can reflect the enhancement of the peroxidative degradation processes involving unsaturated lipids in damaged and aging plant cells.
研究了在离体叶片衰老过程中、落叶植物秋叶以及用敌草快、2,4-二氯苯氧乙酸和2,4,5-三氯苯氧乙酸处理的叶片中积累的脂溶性荧光化合物（LFC）的色谱特性（如薄层迁移率、分子吸附分离和凝胶色谱）和荧光参数。讨论了植物组织中LFC检测和分析的特点。对LFC的各个组分进行了分级分离并比较了它们的性质。将LFC的性质与类脂褐素样动物色素以及在豌豆叶微粒体中由还原吡啶核苷酸刺激的大鼠肝微粒体中NADPH依赖性脂质过氧化系统和分离叶绿体中的脂质光过氧化形成的荧光产物的性质进行了比较。所获得的数据表明，“重”LFC组分的积累可以反映受损和衰老植物细胞中涉及不饱和脂质的过氧化降解过程的增强。
