Rayfield G W
Biophys J. 1982 Apr;38(1):79-84. doi: 10.1016/S0006-3495(82)84533-5.
The short-circuit photoresponse of model membranes containing bacteriorhodopsin to short (35 ms) and long (3.5 s) light pulses is described. It is shown that if the light pulse is short compared with the charging and discharging times of the model membrane, the temporal response of the light-driven proton pump can be measured. Photoactive planar model membranes were formed both from biomolecular lipid membranes and from solid 6-micrometers thick Teflon septa coated with lipid and bacteriorhodopsin. The kinetic response of the pump is independent of the planar model membrane system in which it is incorporated. Experimental evidence indicates that the shape of the leading and trailing edges of the photoresponse curve for the pump deviates from simple exponential behavior. The short-circuit photoresponse of spinach chloroplast in a planar model membrane was also studied for comparison purposes.
描述了含有细菌视紫红质的模型膜对短（35毫秒）和长（3.5秒）光脉冲的短路光响应。结果表明，如果光脉冲与模型膜的充电和放电时间相比很短，则可以测量光驱动质子泵的时间响应。光活性平面模型膜由生物分子脂质膜和涂有脂质和细菌视紫红质的6微米厚的固态聚四氟乙烯隔膜形成。泵的动力学响应与它所包含的平面模型膜系统无关。实验证据表明，泵的光响应曲线的前沿和后沿形状偏离简单的指数行为。为了进行比较，还研究了菠菜叶绿体在平面模型膜中的短路光响应。
