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用于对乙酰氨基酚的快速比色测定法,无阿司匹林或去氧肾上腺素干扰。

Rapid colorimetric assay for acetaminophen without salicylate or phenylephrine interference.

作者信息

Swanson M B, Walters M I

出版信息

Clin Chem. 1982 May;28(5):1171-3.

PMID:7074898
Abstract

A simple rapid, and economical procedure is presented for the quantitative determination of acetaminophen in serum. Acetaminophen is extracted into solvent, and, when back-extracted into base, is simultaneously reacted with Folin-Ciocalteau reagent to form a colored complex. The potential interference of bilirubin is eliminated by treatment with p-diazobenzene sulfonic acid. Results obtained with this method agree with those obtained by "high-pressure" liquid chromatography.

摘要

本文介绍了一种简单、快速且经济的血清中对乙酰氨基酚定量测定方法。对乙酰氨基酚被萃取到溶剂中,再反萃取到碱液中时,会与福林-西奥尔特试剂同时反应形成有色络合物。用对重氮苯磺酸处理可消除胆红素的潜在干扰。该方法所得结果与“高压”液相色谱法所得结果一致。

相似文献

2
Colorimetric determination of acetaminophen.对乙酰氨基酚的比色测定法。
Clin Biochem. 1983 Aug;16(4):220-1. doi: 10.1016/s0009-9120(83)90028-0.
8
Colorimetric assay for acetaminophen in serum.血清中对乙酰氨基酚的比色测定法。
Ther Drug Monit. 1984;6(4):449-53. doi: 10.1097/00007691-198412000-00012.

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