Beck O E, Kaiser P E
Clin Chem. 1982 Jun;28(6):1349-51.
We have developed an immunoassay method for the determination of human IgE concentration in serum by rate nephelometry. Using the Beckman Immunochemistry Analyzer, human IgE antisera, and a human IgE standard, we analyzed 150 serum samples from normal and allergic patients. We report these results and those from comparison studies involving PRIST and Phadebas kits (both from Pharmacia), and Enzygnost (Behringwerke) kits. We also discuss diagnosis of allergy patients and their classification according to their concentrations of IgE and IgG4.
我们已经开发出一种通过速率散射比浊法测定血清中人类IgE浓度的免疫分析方法。使用贝克曼免疫化学分析仪、人类IgE抗血清和人类IgE标准品，我们分析了150份来自正常人和过敏患者的血清样本。我们报告这些结果以及来自涉及PRIST和Phadebas试剂盒（均来自Pharmacia）和Enzygnost（Behringwerke）试剂盒的比较研究的结果。我们还讨论了过敏患者的诊断以及根据他们的IgE和IgG4浓度进行的分类。
