Beck O E, Kaiser P E
Clin Chem. 1981 Feb;27(2):310-3.
An immunoassay method for determination of human IgG subclass concentration by rate nephelometry was developed by using the Beckman Immunochemistry Analyzer, subclass-specific antisera, and a human serum standard. Twelve sera derived from normal as well as gammapathological patients were analyzed. The total IgG concentration, as determined by using a readily available kit, correlated with the sum of the concentration of the individual subclasses. Most of the gammapathological cases were shown to be the IgG1 subclass, which was confirmed by radial immunodiffusion.
采用贝克曼免疫化学分析仪、亚类特异性抗血清和人血清标准品，建立了一种速率散射比浊法测定人IgG亚类浓度的免疫分析方法。对12份来自正常人和γ球蛋白病患者的血清进行了分析。使用市售试剂盒测定的总IgG浓度与各个亚类浓度之和相关。大多数γ球蛋白病病例显示为IgG1亚类，这通过放射免疫扩散得到了证实。
