Bossuyt X, Blanckaert N
Department of Clinical Pathology, University Hospital Leuven, Kapucijnenvoer 33, B-3000 Leuven, Belgium.
Clin Chem. 1999 Jan;45(1):62-7.
We performed interference studies for IgG, IgA, IgM, haptoglobin, and alpha1-antitrypsin assayed in serum, using either fixed-time nephelometry on the BN 100 from Behring or rate nephelometry on two analyzers from Beckman Instruments. For clear serum samples, results for IgG, IgA, IgM, and haptoglobin obtained with the three nephelometers showed good agreement. Values for alpha1-antitrypsin in clear sera were lower with the BN 100 than with the Array 360 or Immage. In lipemic samples, the BN 100 gave higher values than the Array 360 or Immage for all analytes except IgG. Addition of Intralipid to serum produced atypical reactions with the BN 100 (fixed-time nephelometry) but not with the Array 360 or Immage (rate nephelometry). The interference of lipemia on the BN 100 was also seen when the Beckman antibody was used, indicating that the effect was reagent-independent. For hemolyzed samples, the BN 100 gave higher values than the Array 360 or Immage for haptoglobin but not for the other analytes. Addition of increasing amounts of a hemolysate to serum revealed a negative interference in all assay systems. This effect was more pronounced with the Beckman reagent than with the Behring reagent in all three nephelometers and was independent of the type of instrument (fixed-time vs rate nephelometry).
我们使用贝林公司的BN 100进行定时散射比浊法，或贝克曼仪器公司的两种分析仪进行速率散射比浊法，对血清中检测的IgG、IgA、IgM、触珠蛋白和α1抗胰蛋白酶进行了干扰研究。对于清亮血清样本，用这三种散射比浊仪测得的IgG、IgA、IgM和触珠蛋白结果显示出良好的一致性。清亮血清中α1抗胰蛋白酶的值，BN 100测得的低于Array 360或Immage测得的。在脂血样本中，除IgG外，BN 100对所有分析物测得的值均高于Array 360或Immage。向血清中添加脂肪乳剂会使BN 100（定时散射比浊法）产生非典型反应，但不会使Array 360或Immage（速率散射比浊法）产生非典型反应。使用贝克曼抗体时，也观察到脂血对BN 100的干扰，这表明该效应与试剂无关。对于溶血样本，BN 100测得的触珠蛋白值高于Array 360或Immage，但其他分析物并非如此。向血清中添加越来越多的溶血产物显示在所有检测系统中均存在负干扰。在所有三种散射比浊仪中，这种效应在贝克曼试剂中比在贝林试剂中更明显，并且与仪器类型（定时与速率散射比浊法）无关。
