Tsibris J C, Cazenave C R, Cantor B, Notelovitz M, Kalra P S, Spellacy W N
Am J Obstet Gynecol. 1978 Oct 15;132(4):449-54. doi: 10.1016/0002-9378(78)90783-4.
Unoccupied estrogen receptors and progesterone receptors were measured in the cytoplasm of five sections along the length of endometrium obtained from noncancerous, premenopausal hysterectomy specimens. The concentrations of the two receptors were measured with tritiated estradiol or R5020 (a synthetic progestin), the latter two having been purified by high-pressure liquid chromatography, and were found to be highest in the fundus and lowest in the cervix. Progesterone receptor levels, ranging from 50 to 3,500 fmoles of R5020 bound per milligram of protein, were generally much higher in each section of the endometrium than estrogen receptor levels, which ranged from 0 to 500 fmoles of estradiol bound per milligram of protein. Near ovulation it seemed that the distribution profiles of both receptors became very steep, with more than a tenfold difference in the receptor levels being found between the fundus and the cervix. Receptor levels measured in endometrial samples obtained by curettage or aspiration should be interpreted with caution.
在取自非癌性绝经前子宫切除标本的子宫内膜全长的五个切片的细胞质中，检测了未被占据的雌激素受体和孕激素受体。使用经高压液相色谱法纯化的氚标记雌二醇或R5020（一种合成孕激素）测量了这两种受体的浓度，发现其在子宫底部最高，在子宫颈最低。孕激素受体水平为每毫克蛋白质结合50至3500飞摩尔R5020，在子宫内膜的每个切片中通常比雌激素受体水平高得多，雌激素受体水平为每毫克蛋白质结合0至500飞摩尔雌二醇。在接近排卵期时，两种受体的分布曲线似乎变得非常陡峭，子宫底部和子宫颈之间的受体水平相差超过十倍。通过刮宫或抽吸获得的子宫内膜样本中测得的受体水平应谨慎解读。
