Douer D, Koeffler H P
J Clin Invest. 1982 Apr;69(4):1039-41. doi: 10.1172/jci110507.
We studied the effect of retinoic acid on the clonal proliferation of normal human early erythroid progenitor cells in vitro. Normal peripheral blood cells were cultured in methylcellulose with erythropoietin and the number of burst-forming units-erythroid (BFU-E) colonies were scored on day 12 of culture. All-trans retinoic acid increased the number of colonies in a dose-response fashion. Maximal stimulation occurred at 30 nM retinoic acid, which increased the number of BFU-E by a mean of 225 +/- 25% (+/- SE) over plates containing erythropoietin alone. Colony formation increased even in the presence of maximally stimulating concentrations of erythropoietin. The 13-cis retinoic acid stimulated BFU-E proliferation in a parallel manner as the trans analogue, while retinol (vitamin A) did not affect clonal growth. This data supports further the thesis that retinoic acid, in addition to its known effect on epithelial cells, may be involved in the growth of normal hematopoietic cells.
我们在体外研究了视黄酸对正常人早期红系祖细胞克隆增殖的影响。将正常外周血细胞与促红细胞生成素一起在甲基纤维素中培养，并在培养的第12天对红系爆式集落形成单位（BFU-E）集落的数量进行计数。全反式视黄酸以剂量反应方式增加集落数量。在30 nM视黄酸时出现最大刺激，与仅含促红细胞生成素的平板相比，其使BFU-E数量平均增加225±25%（±标准误）。即使在促红细胞生成素的最大刺激浓度存在的情况下，集落形成也增加。13-顺式视黄酸与反式类似物以平行方式刺激BFU-E增殖，而视黄醇（维生素A）不影响克隆生长。该数据进一步支持了这样的论点，即视黄酸除了对上皮细胞有已知作用外，可能还参与正常造血细胞的生长。
