Cohen A B, Rossi M, Geczy D, Knight L
J Clin Invest. 1982 Apr;69(4):794-8. doi: 10.1172/jci110518.
Neutrophil turnover was studied in the blood and alveoli of normal rabbits. Blood neutrophil turnover was examined by two different methods. In the first method, donor rabbit neutrophils were labeled in vivo by injecting tritium-labeled thymidine intravenously. After 72 h recipient rabbits received blood from the donors. The decline of the specific radioactivity of blood neutrophils was used to determine that their half-life was 4.03 h. In the second method, rabbit peritoneal exudative neutrophils were elicited with oyster glycogen. These cells were labeled with 111Indium oxine and infused into the blood of recipient animals. By their decline in specific radioactivity, the half-life of the blood neutrophil was 4.08 h. These half-lives are not significantly different. Lung lavage was performed on the animals that received the 111Indium-labeled neutrophils and the turnover time of the lung neutrophil was found to be 2.63 h. The turnover of the alveolar neutrophil pool accounted for only 0.19% of the total turnover of the blood neutrophils. Therefore, the lung appears to contribute only minimally to the total capacity of the body to dispose of neutrophils.
在正常兔子的血液和肺泡中研究了中性粒细胞的更新情况。通过两种不同方法检测血液中性粒细胞的更新。第一种方法，通过静脉注射氚标记的胸腺嘧啶核苷在体内标记供体兔子的中性粒细胞。72小时后，受体兔子接受来自供体的血液。利用血液中性粒细胞比放射性的下降来确定其半衰期为4.03小时。第二种方法，用牡蛎糖原诱导兔腹膜渗出性中性粒细胞。这些细胞用铟-111 草氨酸标记并注入受体动物的血液中。根据其比放射性的下降，血液中性粒细胞的半衰期为4.08小时。这些半衰期没有显著差异。对接受铟-111标记中性粒细胞的动物进行肺灌洗，发现肺中性粒细胞的更新时间为2.63小时。肺泡中性粒细胞池的更新仅占血液中性粒细胞总更新量的0.19%。因此，肺似乎对机体清除中性粒细胞的总能力贡献极小。
