Roy N K, Ghosh R K, Das J
J Gen Microbiol. 1982 Feb;128(2):349-53. doi: 10.1099/00221287-128-2-349.
The synthesis of alkaline phosphatase by two strains of Vibrio cholerae belonging to the Inaba and Ogawa serotypes has been examined in relation to the phosphate concentration of the culture medium. The synthesis of the enzyme in both strains was repressed in cells grown in the presence of a high concentration of inorganic phosphate. Lowering the phosphate content of the growth medium led to a derepression of enzyme activity. The presence of glucose in low phosphate medium stimulated the degree of derepression. The synthesis of the enzyme by strain Inaba 569B was more sensitive to inorganic phosphate than that of strain Ogawa 154. The enzyme was presumably located in the periplasmic space since it was released when the organisms were converted to spheroplasts.
已针对培养基中的磷酸盐浓度，研究了两株分别属于稻叶型和小川型的霍乱弧菌碱性磷酸酶的合成情况。在高浓度无机磷酸盐存在下生长的细胞中，两株菌中该酶的合成均受到抑制。降低生长培养基中的磷酸盐含量会导致酶活性的去阻遏。低磷酸盐培养基中葡萄糖的存在刺激了去阻遏程度。稻叶型569B菌株的酶合成比小川型154菌株对无机磷酸盐更敏感。该酶可能位于周质空间，因为当菌体转化为原生质球时它会释放出来。
