Shiba T, Hill R T, Straube W L, Colwell R R
Shimonoseki University of Fisheries, Nagata-Honmachi Shimonoseki, Japan.
Appl Environ Microbiol. 1995 Jul;61(7):2583-8. doi: 10.1128/aem.61.7.2583-2588.1995.
The culturability of Vibrio cholerae O1 serotype Inaba strain 569B was decreased by the addition of glucose to cell suspensions in starvation media. A similar effect was observed with sucrose, maltose, and fructose. We term this inhibitory effect glucose shock. It was not observed with arabinose or xylose or with carboxylates, such as acetate and pyruvate. No acidification of the medium occurred in the presence of these carbohydrates. Glucose shock was prevented by the addition of nitrogen or phosphorus sources. In the presence of phosphate, the bacterium produced formic acid from glucose. The phenomenon of glucose shock was also observed in V. cholerae O1 serotype Inaba strain RIMD 2203082 but not in strain RIMD 2203088 (O1 Inaba), IID 936 (O1 Ogawa), or RIMD 2214034 (non-O1). The culturability of Escherichia coli, Enterobacter aerogenes, and Listonella anguillarum did not decrease in starvation media with added glucose. Hence, the phenomenon should have ecological significance in determining the distribution of bacteria in marine ecosystems in situations where carbohydrates are abundant, but nitrogen and phosphorus are limiting.
在饥饿培养基中，向霍乱弧菌O1群稻叶型菌株569B的细胞悬液中添加葡萄糖会降低其可培养性。蔗糖、麦芽糖和果糖也有类似效果。我们将这种抑制作用称为葡萄糖休克。阿拉伯糖、木糖或羧酸盐（如乙酸盐和丙酮酸盐）则未观察到这种现象。在这些碳水化合物存在的情况下，培养基不会酸化。添加氮源或磷源可防止葡萄糖休克。在有磷酸盐存在的情况下，该细菌会从葡萄糖产生甲酸。在霍乱弧菌O1群稻叶型菌株RIMD 2203082中也观察到了葡萄糖休克现象，但在菌株RIMD 2203088（O1稻叶型）、IID 936（O1小川型）或RIMD 2214034（非O1群）中未观察到。在添加葡萄糖的饥饿培养基中，大肠杆菌、产气肠杆菌和鳗弧菌的可培养性并未降低。因此，在碳水化合物丰富但氮和磷有限的情况下，这种现象对于确定海洋生态系统中细菌的分布应该具有生态意义。
