Wiencke J K, Vosika J, Johnson P, Wang N, Garry V F
Pharmacology. 1982;24(2):67-73. doi: 10.1159/000137578.
Three carcinogens with different requirements for activation were used to generate sister chromatid exchange (SCE) in cultured lymphocytes from 22 untreated patients with solid tumors. These data were compared to SCEs produced by incubating lymphocytes, from 44 normal persons, with the same carcinogens. In vitro treatments with butadiene epoxide (0.125 micrograms/ml) show no significant differences in the SCEs generated. However, in vitro treatment with mitomycin-C (0.04 micrograms/ml) did induce significant differences (p less than 0.05) in the distribution of the SCE scores. Treatment of the cultured cells with benzo(a)pyrene (10 micrograms/ml), a carcinogen requiring extensive metabolic activation, significantly (p less than 0.001) altered the distribution of the SCEs generated in vitro. The SCE data suggest that metabolic activities play an important role in the processing of carcinogens in solid tumor patients.
使用三种对激活有不同要求的致癌物，在22名未经治疗的实体瘤患者的培养淋巴细胞中产生姐妹染色单体交换（SCE）。将这些数据与44名正常人的淋巴细胞与相同致癌物孵育产生的SCE进行比较。用1,2-环氧丁烷（0.125微克/毫升）进行体外处理，所产生的SCE没有显著差异。然而，用丝裂霉素-C（0.04微克/毫升）进行体外处理确实在SCE分数分布上诱导出显著差异（p小于0.05）。用苯并(a)芘（10微克/毫升）处理培养细胞，苯并(a)芘是一种需要广泛代谢激活的致癌物，显著（p小于0.001）改变了体外产生的SCE的分布。SCE数据表明，代谢活性在实体瘤患者致癌物的处理过程中起重要作用。
