Immediate effect of ultraviolet-B radiation on epidermal lysosomal hydrolases.

The immediate effect of ultraviolet-B radiation on epidermal lysosomal enzymes in vivo was studied by irradiating the roof of suction blisters. The activities of ten lysosomal hydrolases were determined in the blister fluid and in the epidermal homogenates. The enzyme values were decreased in the irradiated epidermis compared with the non-irradiated epidermis of control suction blisters, but the values found in the fluid of irradiated blisters were not decreased compared with control fluid.