Glynn P, Gilbert H M, Newcombe J, Cuzner M L
Clin Exp Immunol. 1982 Apr;48(1):102-10.
Immunoglobulin G (IgG) in soluble and particulate fractions of discrete tissue samples from multiple sclerosis (MS) brain was analysed. Supernatant IgG/albumin rations and particulate-bound IgG levels were highest in samples dissected from MS plaques and adjacent white matter. Acid extracts of particulate fractions from the equivalent of 1 g of MS plaque tissue contained up to 15 micrograms IgG, an order of magnitude less than the amount in extracts from a subacute sclerosing panencephatitis (SSPE) brain, but 20 times more than those from control brain. By contrast, supernatant fractions from SSPE brain and some MS plaques contained comparable amounts (100-200 micrograms/g tissue) of IgG, which were 10 times greater than those from control brain. Samples were subjected to isoelectric focusing (IEF), and IgG was visualized by immunoperoxidase staining. The IEF patterns of IgG from control samples were diffuse but samples from demyelinated MS tissue displayed distinct oligoclonal bands of IgG. A number of common IgG bands were apparent in extracts of supernatant and particulate fractions from the same plaque. The IEF spectra of plaque samples from three MS brains were different. Furthermore, quantitative variations in certain IgG bands were observed in different plaques from the same brain.
对来自多发性硬化症（MS）脑部离散组织样本的可溶性和颗粒部分中的免疫球蛋白G（IgG）进行了分析。从MS斑块和相邻白质中分离出的样本中，上清液IgG/白蛋白比值和颗粒结合IgG水平最高。相当于1克MS斑块组织的颗粒部分的酸提取物中含有多达15微克的IgG，比亚急性硬化性全脑炎（SSPE）脑提取物中的量少一个数量级，但比对照脑提取物中的量多20倍。相比之下，SSPE脑和一些MS斑块的上清液部分含有相当数量（100 - 200微克/克组织）的IgG，比对照脑中的量高10倍。对样本进行等电聚焦（IEF），并通过免疫过氧化物酶染色观察IgG。对照样本的IgG的IEF图谱是弥散的，但脱髓鞘MS组织的样本显示出明显的IgG寡克隆带。来自同一斑块的上清液和颗粒部分提取物中出现了一些常见的IgG带。来自三个MS脑的斑块样本的IEF光谱不同。此外，在同一脑的不同斑块中观察到某些IgG带的定量变化。
