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一种具有短光路和氧电极室的反应池,用于方便地定量测定血红蛋白与氧的结合情况。

A reaction cell with short optical path and oxygen electrode compartment for convenient quantitative determination of oxygen binding by hemoglobin.

作者信息

Horiuchi K, Kaneko S, Asai H

出版信息

Int J Biochem. 1982;14(5):359-62. doi: 10.1016/0020-711x(82)90019-2.

DOI:10.1016/0020-711x(82)90019-2
PMID:7084554
Abstract
  1. A simple and compact apparatus, with which the oxygen equilibrium of hemoglobin can be determined easily, was constructed and its performance was examined. 2. The oxygen partial pressure and the degree of oxygen saturation in a hemoglobin solution were determined by combined used of a Clark oxygen electrode and a spectrophotometer. 3. This apparatus can be used in a usual 1 cm square cell holder of a commercial spectrophotometer without any modifications, and has the advantage that the light path length of the optical cell is variable and can be shortened to about 2 mm.
摘要
  1. 构建了一种简单紧凑的装置,利用该装置可轻松测定血红蛋白的氧平衡,并对其性能进行了检测。2. 通过联合使用克拉克氧电极和分光光度计来测定血红蛋白溶液中的氧分压和氧饱和度。3. 该装置无需任何改装即可用于商用分光光度计常见的1平方厘米样品池架中,其优点是光学样品池的光程长度可变,可缩短至约2毫米。

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