Lindmark D G, Jarroll E L, Timoney P J, Shin S J
Infect Immun. 1982 May;36(2):531-4. doi: 10.1128/iai.36.2.531-534.1982.
The energy metabolism of the English E-CMO strain of contagious equine metritis bacterium was studied in whole cells and cell extracts. This bacterium appears to have an active Krebs cycle and probably obtains energy by oxidative phosphorylation since glycolysis and the hexose monophosphate pathways appear to be absent. These conclusions are based on the findings that [U-14C]glucose incorporation by this bacterium is below the level of detection, and that respiration is stimulated by Krebs cycle intermediates (i.e., malate, citrate, and succinate), but not by glucose, fructose, maltose, or sucrose. Furthermore, support comes from the fact that enzymes generally associated with the Krebs cycle and electron transport (i.e., malate dehydrogenase, succinate dehydrogenase, isocitrate dehydrogenase, fumarate hydratase, malate dehydrogenase [decarboxylating], cytochrome oxidase, superoxide dismutase, NADH dehydrogenase, and catalase) were detected. Those enzymes normally associated with glycolysis and the hexose monophosphate pathways (i.e., hexokinase, glucose 6-phosphate dehydrogenase, fructose biphosphate aldolase, glycerol 3-phosphate dehydrogenase, phosphoenolpyruvate carboxykinase, pyruvate kinase, phosphate acetyl transferase, acetate kinase, alcohol dehydrogenase, and lactate dehydrogenase) were below the level of detection.
对马传染性子宫炎细菌英国E - CMO菌株的能量代谢在全细胞和细胞提取物中进行了研究。该细菌似乎具有活跃的三羧酸循环，并且可能通过氧化磷酸化获取能量，因为糖酵解和磷酸戊糖途径似乎不存在。这些结论基于以下发现：该细菌对[U - 14C]葡萄糖的摄取低于检测水平，并且呼吸作用受到三羧酸循环中间产物（即苹果酸、柠檬酸和琥珀酸）的刺激，但不受葡萄糖、果糖、麦芽糖或蔗糖的刺激。此外，有证据支持这一观点，即检测到了通常与三羧酸循环和电子传递相关的酶（即苹果酸脱氢酶、琥珀酸脱氢酶、异柠檬酸脱氢酶、延胡索酸水合酶、脱羧苹果酸脱氢酶、细胞色素氧化酶、超氧化物歧化酶、NADH脱氢酶和过氧化氢酶）。而那些通常与糖酵解和磷酸戊糖途径相关的酶（即己糖激酶、葡萄糖6 - 磷酸脱氢酶、果糖二磷酸醛缩酶、3 - 磷酸甘油脱氢酶、磷酸烯醇丙酮酸羧激酶、丙酮酸激酶、磷酸乙酰转移酶、乙酸激酶、乙醇脱氢酶和乳酸脱氢酶）低于检测水平。
