Ramentol-Massana M, Grau G, Beaumariage M L, Salmon J
Int Arch Allergy Appl Immunol. 1982;68(3):219-21. doi: 10.1159/000233101.
The levels of the C3 breakdown product C3d were measured in plasma, serum, and synovial fluid by the technique of rocket immunoelectrophoresis. Serum and plasma from 22 healthy donors, 4 patients with rheumatoid arthritis, 1 patient with systemic lupus erythematosus, and 1 patient with cryoglobulinemia were used. The synovial fluid was studied in 3 other patients with rheumatoid arthritis. Normal values for the 22 healthy donors in plasma were within a narrow range of 3.25--5.75% as compared to the standard pool that was prepared. Storage of plasma to -80 degrees C during 7--15 days did not modify the results of the test. Significant differences in C3d values were observed between serum and EDTA plasma samples. Patients with immunological diseases showed higher values of C3d in the samples (from 5 to 30% of activity as compared to the standard pool). Measurement of C3d by rocket immunoelectrophoresis showed to be an easy, satisfactory and reliable procedure able to be incorporated to routine immunological evaluations.
采用火箭免疫电泳技术测定血浆、血清和滑液中补体3（C3）裂解产物C3d的水平。使用了来自22名健康供体、4名类风湿关节炎患者、1名系统性红斑狼疮患者和1名冷球蛋白血症患者的血清和血浆。对另外3名类风湿关节炎患者的滑液进行了研究。与制备的标准混合液相比，22名健康供体血浆中的正常数值在3.25%至5.75%的狭窄范围内。在-80℃下储存7至15天的血浆不会改变检测结果。血清和乙二胺四乙酸（EDTA）血浆样本的C3d值存在显著差异。免疫疾病患者样本中的C3d值更高（与标准混合液相比，活性为5%至30%）。通过火箭免疫电泳测定C3d是一种简便、令人满意且可靠的方法，可纳入常规免疫学评估。
