Lambert M W, McGuire J
J Invest Dermatol. 1982 Jun;78(6):498-502. doi: 10.1111/1523-1747.ep12510312.
Histones and 4 nuclear nonhistone protein fractions (NHP1-4) were extracted from nuclei of a Cloudman mouse melanoma cell line (NCTC 3960, CCL 53) and tested for their ability to bind to DNA and influence transcription. The histones and NHP fractions showed different binding affinities for DNA, with the histones and NHP1 exhibiting the highest affinity. The NHP fractions differentially affected both the rate of RNA synthesis and the size of RNA transcribed. NHP1 which inhibited RNA synthesis to the greatest extent, inhibited synthesis of all sizes of RNA except for major peaks of 28S and 8S RNS and discrete minor peaks of 7S, 6S, 5S, and 4S RNS. Histones markedly enhanced the effect of NHP1 on RNA synthesis. These results suggest that there are nonhistone proteins in Cloudman melanoma nuclei which have a high affinity for DNA and which may be involved in the regulation of transcription.
从Cloudman小鼠黑色素瘤细胞系（NCTC 3960，CCL 53）的细胞核中提取组蛋白和4种核非组蛋白组分（NHP1 - 4），并检测它们与DNA结合以及影响转录的能力。组蛋白和NHP组分对DNA表现出不同的结合亲和力，其中组蛋白和NHP1的亲和力最高。NHP组分对RNA合成速率和转录的RNA大小有不同影响。NHP1在最大程度上抑制RNA合成，除了28S和8S RNS的主峰以及7S、6S、5S和4S RNS的离散小峰外，抑制所有大小RNA的合成。组蛋白显著增强了NHP1对RNA合成的影响。这些结果表明，Cloudman黑色素瘤细胞核中存在对DNA具有高亲和力且可能参与转录调控的非组蛋白。
