Quinn P, Whittingham D G, Stanger J D
Arch Androl. 1982 May;8(3):189-98. doi: 10.3109/01485018208987039.
Spermine had a beneficial effect on the fertilization of zona-free mouse ova in vitro. A deleterious effect of spermine or seminal plasma on gametes only occurred if normal, untreated BSA was present in the incubation medium. This was due to the activity of spermine oxidase, which could be assayed in the untreated BSA and which was removed by TCA-purification of the BSA. The addition of a spermine oxidase inhibitor, isoniazid, or the use of the TCA-purified BSA preparation allowed the direct effects of spermine or seminal plasma on mammalian fertilization to be assessed. Human seminal plasma retarded the acquisition of fertilizing ability in human spermatozoa and prevented it in mouse spermatozoa. The fertilizing ability of the spermatozoa of both species was unaffected, when spermatozoa were preincubated to achieve capacitation before adding the seminal plasma.
精胺对体外无透明带小鼠卵子的受精有有益作用。仅当孵育培养基中存在正常、未处理的牛血清白蛋白（BSA）时，精胺或精浆才会对配子产生有害影响。这是由于精胺氧化酶的活性，该酶可在未处理的BSA中检测到，并可通过三氯乙酸（TCA）纯化BSA将其去除。添加精胺氧化酶抑制剂异烟肼或使用经TCA纯化的BSA制剂，可以评估精胺或精浆对哺乳动物受精的直接影响。人类精浆会延缓人类精子获得受精能力，并阻止小鼠精子获得受精能力。当在添加精浆之前对精子进行预孵育以使其获能时，两种物种精子的受精能力均不受影响。
