Davies B D, Hoss W, Lin J P, Lionetti F
Mol Cell Biochem. 1982 Apr 16;44(1):23-31. doi: 10.1007/BF00573842.
A noncholinergic nicotine receptor on human phagocytic leukocytes has been characterized using the binding of 3H-(d,1)-nicotine. The average affinity +/- standard deviation of (d,1)-nicotine for the receptor on neutrophils is 36 +/- 18 nM (n = 6). The binding is saturable with an average of 8.7 x 10(4) sites per neutrophil. Monocytes and to a lesser extent lymphocytes but not erythrocytes also display specific binding. Bound nicotine is dissociable from the receptor and is not metabolized. Only close structural analogs of nicotine bind to the receptor, which is stereoselective for the (d)-isomer. The receptor can be occupied by (1)-nicotine at concentrations present in the blood of smokers. It is suggested that some of the adverse effects of smoking on leukocyte functions may be mediated by a specific nicotine receptor.
利用³H-（d，l）-尼古丁的结合作用，对人吞噬性白细胞上的一种非胆碱能尼古丁受体进行了特性描述。（d，l）-尼古丁对嗜中性粒细胞上该受体的平均亲和力±标准差为36±18 nM（n = 6）。这种结合具有饱和性，每个嗜中性粒细胞平均有8.7×10⁴个位点。单核细胞以及程度稍轻的淋巴细胞也表现出特异性结合，但红细胞没有。结合的尼古丁可从受体上解离，且不会被代谢。只有尼古丁的紧密结构类似物能与该受体结合，该受体对（d）-异构体具有立体选择性。吸烟者血液中存在的浓度的（l）-尼古丁就能占据该受体。有人提出，吸烟对白细胞功能的一些不良影响可能是由一种特定的尼古丁受体介导的。
