Robert K H, Gahrton G, Friberg K, Zech L, Nilsson B
Scand J Haematol. 1982 Feb;28(2):163-8. doi: 10.1111/j.1600-0609.1982.tb00509.x.
Peripheral blood lymphocytes from 22 consecutive patients with chronic lymphocytic leukaemia were stimulated with the polyclonal B-cell mitogens lipopolysaccharide from E. coli (LPS) and Epstein-Barr virus (EBV). Stimulation was successful for chromosome analysis in 14 patients. Eleven patients had chromosomal aberrations and 7 of these had an extra chromosome 12. In 2 patients an extra chromosome 12 was the only abnormality, while additional aberrations were found in 5 patients. 3 patients had complex aberrations involving deletion of chromosome 6. 1 of these patients also had a translocation between chromosomes 12 and 14. 1 patient had a translocation between chromosomes 11 and 14. In 3 patients no aberrations were detected. The time elapsing between diagnosis and appearance of clinical symptoms which were indications for treatment was significantly shorter in patients with an extra chromosome 12 than in these without this abnormality. Thus, it appears that an extra chromosome 12 is associated with a more rapid course of the disease, and may therefore be of importance for the predition of prognosis.
用来自大肠杆菌的多克隆B细胞有丝分裂原脂多糖（LPS）和爱泼斯坦-巴尔病毒（EBV）刺激22例连续性慢性淋巴细胞白血病患者的外周血淋巴细胞。14例患者的刺激结果成功用于染色体分析。11例患者有染色体畸变，其中7例有额外的12号染色体。2例患者中额外的12号染色体是唯一异常，而另外5例患者还发现有其他畸变。3例患者有涉及6号染色体缺失的复杂畸变。其中1例患者还存在12号和14号染色体之间的易位。1例患者有11号和14号染色体之间的易位。3例患者未检测到畸变。有额外12号染色体的患者从诊断到出现作为治疗指征的临床症状的时间明显短于无此异常的患者。因此，似乎额外的12号染色体与疾病进展更快相关，因此可能对预后预测具有重要意义。
