Odushko N P
Vopr Pitan. 1982 Mar-Apr(2):52-6.
The blood serum of white rats kept on atherogenic diet for 7 days did not show any changes as regards the correlation of lipids separated by thin-layer chromatography. Significantly reduced was only the proportion of phospholipids. Liver cell mitochondria demonstrated an increase in the relative content of cholesterol esters but a lowering of the content of triglycerides and free cholesterol. The microsomes demonstrated an increase in the relative content of cholesterol esters with a decline in the content of free fatty acids. Of cholesterol esters, the rise in the percentage of cholesteryl oleate and cholesterol esters with saturated fatty acids was proportional both in the blood serum and subcellular fractions of the liver. Fourteen and 28 days after keeping the animals on the atherogenic diet these changes became more manifest. The blood serum showed a decrease in the content of cholesteryl arachidonate with no changes in the content of cholesteryl linoleate. At the same time there was no parallelism in the changes in the content of cholesteryl arachidonate and cholesteryl linoleate in the blood serum, mitochondria or microsomes of liver cells. It is concluded that the changes in the liver lipid composition may be characterized most precisely by the relative content of blood serum cholesteryl oleate and cholesterol esters with saturated fatty acids.
喂食致动脉粥样化饮食7天的白鼠血清，就薄层色谱法分离出的脂质相关性而言未显示任何变化。仅磷脂比例显著降低。肝细胞线粒体显示胆固醇酯相对含量增加，但甘油三酯和游离胆固醇含量降低。微粒体显示胆固醇酯相对含量增加，游离脂肪酸含量下降。在血清和肝脏亚细胞组分中，油酸胆固醇酯和含饱和脂肪酸的胆固醇酯百分比的上升是成比例的。在给动物喂食致动脉粥样化饮食14天和28天后，这些变化变得更加明显。血清中花生四烯酸胆固醇酯含量降低，亚油酸胆固醇酯含量无变化。同时，血清、肝细胞线粒体或微粒体中花生四烯酸胆固醇酯和亚油酸胆固醇酯含量的变化不存在平行关系。结论是，肝脏脂质组成的变化最精确地可用血清油酸胆固醇酯和含饱和脂肪酸的胆固醇酯的相对含量来表征。
