Ruben F L, Holzman I R, Fireman P
Am J Obstet Gynecol. 1982 Jul 1;143(5):518-22. doi: 10.1016/0002-9378(82)90540-3.
Paired samples of peripheral blood and colostrum or milk were obtained from women 1 to 6 days after delivery. After Ficoll-Hypaque centrifugation, peripheral blood lymphocytes (PBL) and colostrum or milk lymphocytes (COL) were examined for surface membrane characteristics and in vitro proliferative reactivity. As determined by sheep red blood cell rosetting and by immunoglobulin-coated beads, PBL showed an average of 54% T and 12% B lymphocytes, whereas COL showed 33% T and 22% B rosettes, respectively. Paired samples of PBL and COL were used for lymphocyte transformation with three distinct strains of influenza virus: A/USSR, A/Victoria, and B/Hong Kong. Of COL from nine subjects tested against all three strains of influenza virus, five samples (56%) gave positive responses to at least one. These studies indicate that COL contain cells responsive in vitro to several influenza antigens. Such sensitized cells may provide a mechanism for the transfer of antigen responsiveness and protection from the mother to the neonate.
在分娩后1至6天从女性体内获取外周血与初乳或乳汁的配对样本。经Ficoll-泛影葡胺离心后，对外周血淋巴细胞（PBL）和初乳或乳汁淋巴细胞（COL）进行表面膜特征及体外增殖反应性检测。通过绵羊红细胞玫瑰花结试验和免疫球蛋白包被珠检测确定，PBL平均含有54%的T淋巴细胞和12%的B淋巴细胞，而COL分别显示33%的T玫瑰花结和22%的B玫瑰花结。使用PBL和COL的配对样本，用三种不同株系的流感病毒进行淋巴细胞转化试验：A/苏联株、A/维多利亚株和B/香港株。在针对所有三种流感病毒株进行检测的九名受试者的COL样本中，五个样本（56%）对至少一种病毒株呈阳性反应。这些研究表明，COL含有在体外对几种流感抗原产生反应的细胞。此类致敏细胞可能为抗原反应性从母亲向新生儿的传递及保护提供一种机制。
