Bey R F, Johnson R C
Am J Vet Res. 1982 May;43(5):831-4.
The immunogenicity of leptospires cultivated in modified bovine albumin-polysorbate 80 medium and those cultivated in protein-free medium were quantitatively evaluated in the dog. Vaccine preparations, whole cell or outer envelope, prevented leptospiremia; however, kidney culture data revealed that 1 of 4 dogs vaccinated with 0.1 to 1 mg of whole cell prepared from leptospires cultivated in the modified bovine albumin-polysorbate 80 medium was positive for Leptospira, whereas dogs vaccinated with whole cells prepared in protein-free medium were not. Dogs vaccinated with greater than or equal to 0.5 mg of outer envelope were refractory to infection after challenge exposure.
在犬类中对在改良牛白蛋白 - 聚山梨醇酯80培养基中培养的钩端螺旋体以及在无蛋白培养基中培养的钩端螺旋体的免疫原性进行了定量评估。疫苗制剂，全细胞或外膜，可预防钩端螺旋体血症；然而，肾脏培养数据显示，用在改良牛白蛋白 - 聚山梨醇酯80培养基中培养的钩端螺旋体制备的0.1至1毫克全细胞对4只接种犬中的1只呈钩端螺旋体阳性，而用无蛋白培养基中制备的全细胞接种的犬则未出现阳性。接种大于或等于0.5毫克外膜的犬在激发暴露后对感染具有抵抗力。
