Gaffin S L, Badsha N, Brock-Utne J G, Vorster B J, Conradie J D
Ann Clin Biochem. 1982 May;19(3):191-4. doi: 10.1177/000456328201900313.
We report an ELISA method suitable for the large-scale screening of blood bank stores to identify those blood units containing high concentrations of antiendotoxin antibodies. In Natal, 8.3% of total units collected had antiendotoxin antibodies at concentrations greater than 40 micrograms/ml, values that may be therapeutically useful. We found that one technician could screen enough samples per year to produce 800 litres of such high-titre plasma.
我们报告了一种酶联免疫吸附测定(ELISA)方法,该方法适用于大规模筛查血库库存,以识别那些含有高浓度抗内毒素抗体的血液单位。在纳塔尔,所采集的全部血液单位中有8.3%含有浓度高于40微克/毫升的抗内毒素抗体,这些数值可能具有治疗作用。我们发现一名技术人员每年能够筛查足够多的样本,以生产800升这种高滴度血浆。