Rabson J A, Geyer S J, Levine G, Swartz W M, Futrell J W
Ann Surg. 1982 Jul;196(1):92-9. doi: 10.1097/00000658-198207000-00019.
Inguinal lymph nodes in the Buffalo rat were autotransplanted to the popliteal fossa by an intact vascular pedicle or by microvascular anastomosis. These revascularized nodes had normal histology and made spontaneous afferent and efferent lymphatic reconnection with surrounding lymphatic vessels, as documented by ink and silicone rubber injection studies. Lymphoscintigraphy with 99mTc antimony sulfide colloid correctly predicted the 44 of 120 node transplants that had made afferent reconnection. To demonstrate immunologic activity of lymph nodes following transplantation, a cellular adherance assay was employed to detect cell-mediated cytotoxicity of lymph node cells isolated from rats sensitized to an allogeneic gliosarcoma. Cytotoxicity was detected in nontransplanted regional nodes sensitized to tumor (p less than 0.01) and in nodes transplanted by vascular pedicle and then sensitized to tumor (p less than 0.001). This study demonstrates that lymph nodes can be transplanted with restoration of functional lymphatic anatomy, and that following transplantation, lymph nodes retain the ability to mount an immune response against tumor.
将水牛大鼠的腹股沟淋巴结通过完整的血管蒂或微血管吻合术自体移植至腘窝。这些血管再通的淋巴结组织学正常，并与周围淋巴管自发建立了传入和传出淋巴管连接，墨水和硅橡胶注射研究证实了这一点。用99mTc硫化锑胶体进行的淋巴闪烁造影正确预测了120例淋巴结移植中有44例实现了传入连接。为了证明移植后淋巴结的免疫活性，采用细胞黏附试验检测从对同种异体胶质肉瘤致敏的大鼠中分离出的淋巴结细胞的细胞介导细胞毒性。在对肿瘤致敏的未移植区域淋巴结（p<0.01）以及通过血管蒂移植然后对肿瘤致敏的淋巴结（p<0.001）中检测到细胞毒性。本研究表明，淋巴结移植后可恢复功能性淋巴解剖结构，且移植后淋巴结保留了对肿瘤产生免疫反应的能力。
