Ertürk M, Jennings R, Oxley K M, Hastings M J
Department of Virology, Medical School, University of Sheffield, UK.
Med Microbiol Immunol. 1989;178(4):199-209. doi: 10.1007/BF00202553.
The effect of various strains of influenza virus on polymorphonuclear leucocyte (PMNL) function were studied by chemiluminescence (CL) and bacterial killing assays. All virus strains induced PMNL CL and peak CL correlated with haemagglutination (HA) but not neuraminidase (NA) activity of virus pools. Heat-treatment of virus pools generally had little effect on HA activity or ability to generate a PMN CL response but almost completely destroyed NA activity. Exposure of PMNL to each of the six virus strains resulted in loss of surface-associated sialic acid and a marked depression in both zymosan-induced PMNL CL and PMNL bactericidal capacity. However, there was no correlation between the degree of PMNL functional impairment and virus NA activity and, furthermore, heat treatment of virus pools removed NA activity but generally had little effect on their ability to reduce PMNL function. NA does not appear to play a primary role in impairment of PMNL function by influenza virus.
通过化学发光（CL）和细菌杀伤试验研究了各种流感病毒株对多形核白细胞（PMNL）功能的影响。所有病毒株均诱导PMNL产生CL，且CL峰值与病毒株的血凝（HA）活性相关，但与神经氨酸酶（NA）活性无关。病毒株的热处理通常对HA活性或产生PMN CL反应的能力影响不大，但几乎完全破坏了NA活性。将PMNL暴露于六种病毒株中的每一种都会导致表面相关唾液酸的丢失，以及酵母聚糖诱导的PMNL CL和PMNL杀菌能力的显著降低。然而，PMNL功能受损程度与病毒NA活性之间没有相关性，此外，病毒株的热处理消除了NA活性，但通常对其降低PMNL功能的能力影响不大。NA似乎在流感病毒损害PMNL功能中不发挥主要作用。
