Johannessen J V, Sobrinho-Simões M
Diagn Histopathol. 1982 Apr-Jun;5(2):113-27.
Previous ultrastructural studies on follicular thyroid carcinomas present conflicting results, in particular with regard to the surface features of the neoplastic cells. The present study of 9 cases with varying degrees of differentiation is the first comprehensive investigation by concurrent transmission and scanning electron microscopy. In the well-differentiated tumours we found a slightly increased number of microvilli and a decreased number of cilia per cell as compared to the normal thyroid follicles, while moderately differentiated tumours had a smaller number of microvilli and no cilia. The cells in anaplastic foci had unimpressive surfaces with only scattered clusters of short microvilli. Neither scanning nor transmission electron microscopy seem to be of major importance in the diagnostic evaluation of the individual case of follicular thyroid carcinoma or in the assessment of prognosis. Scanning electron microscopy presents a wealth of information on the three dimensional surface arrangement of the neoplastic cells. However, in a tumour where surface alterations may reflect both changes in endocrine function and malignant potential, the interpretation of isolated morphological findings is extremely difficult.
以往对滤泡状甲状腺癌的超微结构研究结果相互矛盾，尤其是在肿瘤细胞的表面特征方面。本研究对9例不同分化程度的病例进行了首次同时采用透射电子显微镜和扫描电子显微镜的全面调查。与正常甲状腺滤泡相比，在高分化肿瘤中，我们发现每个细胞的微绒毛数量略有增加，纤毛数量减少，而中分化肿瘤的微绒毛数量较少且无纤毛。间变灶中的细胞表面平淡，只有散在的短微绒毛簇。扫描电子显微镜和透射电子显微镜在滤泡状甲状腺癌个体病例的诊断评估或预后评估中似乎都不具有重要意义。扫描电子显微镜提供了关于肿瘤细胞三维表面排列的丰富信息。然而，在一个表面改变可能反映内分泌功能变化和恶性潜能的肿瘤中，对孤立形态学发现的解释极其困难。
