Hirato K, Suzuki T, Simizu A, Hidaka T, Yanaihara T, Nakayama T, Oshima H, Kanbegawa A
Nihon Naibunpi Gakkai Zasshi. 1982 Mar 20;58(3):177-83. doi: 10.1507/endocrine1927.58.3_177.
A radioimmunoassay using specific antisera against 5-androstene-3 beta, 17 beta-diol-11 alpha-succinoyloxy-BSA was developed, and serum concentrations of 5-androstene-3 beta, 17 beta-diol (AD) in normal men and women (maternal vein during pregnancy and cord blood at delivery) were measured. The reliability of the method was assessed by means of the recovery rate (97-109%) and the co-efficient of variation of inter- and intra assay (10-14%). The concentrations in serum samples obtained from various sources were as follows (ng/ml of free steroid mean +/- SD): spermatic vein, 48.57 +/- 50.64, peripheral vein in men aged 57-84, 1.24 +/- 0.47; ovarian vein during follicular phase, 3.81 +/- 3.27, peripheral vein, 1.03 +/- 0.44; maternal vein at delivery, 3.03 +/- 0.77, umbilical artery, 6.58 +/- 3.00, umbilical vein, 6.32 +/- 2.82. Plasma AD levels in the spermatic and ovarian vein were significantly higher than in the peripheral vein, suggesting the gonadal secretion of this steroid. Maternal serum AD concentration increased as pregnancy advanced, and AD levels in cord blood at delivery were significantly higher than that in maternal blood. These results also indicate that AD is one of the products of the feto-placental unit.
我们开发了一种放射免疫分析法，该方法使用针对5-雄烯-3β，17β-二醇-11α-琥珀酰氧基-牛血清白蛋白的特异性抗血清，并测定了正常男性和女性（孕期母血和分娩时脐血）血清中5-雄烯-3β，17β-二醇（AD）的浓度。通过回收率（97-109%）以及批内和批间变异系数（10-14%）评估了该方法的可靠性。从不同来源获得的血清样本中的浓度如下（游离类固醇的ng/ml平均值±标准差）：精索静脉，48.57±50.64；57-84岁男性的外周静脉，1.24±0.47；卵泡期卵巢静脉，3.81±3.27，外周静脉，1.03±0.44；分娩时母血，3.03±0.77，脐动脉，6.58±3.00，脐静脉，6.32±2.82。精索静脉和卵巢静脉中的血浆AD水平显著高于外周静脉，提示该类固醇的性腺分泌。孕期母血AD浓度随孕周增加而升高，分娩时脐血中的AD水平显著高于母血中的水平。这些结果还表明AD是胎儿-胎盘单位的产物之一。
