Fukabori Y
Department of Urology, School of Medicine, Gunma University, Maebashi, Japan.
Nihon Naibunpi Gakkai Zasshi. 1988 May 20;64(5):340-53. doi: 10.1507/endocrine1927.64.5_340.
5 alpha-Androstane-3 alpha, 17 beta-diol (A3 alpha diol) is a potent androgen, and is an end product of testosterone. Many authors measured A3 alpha diol levels in human plasma by various methods, but the levels of this steroid were very dissimilar. In order to validate such values, it was measured by gas chromatography-selected ion monitoring (GC-SIM) in this study. A3 alpha diol, 5 alpha-Androstane-3 beta, 17 beta-diol (A3 beta diol) and Testosterone (T) in human peripheral serum were measured by GC-SIM at the same time. The TFA-derivatives of these compounds were analyzed after purification of the serum extract by Sephadex LH-20 microcolumn chromatography. The sensitivity was good: (16.7 pg/ml: A3 alpha diol, 26.7 pg/ml: A3 beta diol). The precision (CV = 2.75%: A3 alpha diol, 3.11%: A3 beta diol) and the accuracy were better than ever reported. Serum A3 alpha diol was measured in 131 healthy men aged 15-81 years and 5 healthy women aged 25-60 years. There were remarkable differences between individuals in the serum levels of A3 alpha diol, but the levels in male serum (greater than 20y) showed a significant negative correlation with age (r = -0.560, p less than 0.01). When these healthy men were classified into three age groups of 20-39, 40-59 and 60-79 years, the values (mean +/- SD) for serum A3 alpha diol were 189.3 +/- 77.7 (n = 20), 127.9 +/- 59.5 (n = 28), and 94.9 +/- 52.9 (n = 73) pg/ml, respectively. There were significant differences between the levels of this steroid in all age groups (p less than 0.01). There was a weak but significant correlation between serum A3 alpha diol and T levels (r = 0.3235, p less than 0.01) in healthy men (25-77 years, n = 77). Determination of serum A3 alpha diol was influenced by age. The number of samples strongly influenced the decision of mean value of A3 alpha diol levels. These results suggested that these factors had to be made obvious when this steroid was studied.
5α-雄甾烷-3α,17β-二醇（A3α二醇）是一种强效雄激素，是睾酮的终产物。许多作者用各种方法测量人血浆中的A3α二醇水平，但这种类固醇的水平差异很大。为了验证这些值，本研究采用气相色谱-选择离子监测（GC-SIM）法进行测量。同时用GC-SIM法测量人外周血清中的A3α二醇、5α-雄甾烷-3β,17β-二醇（A3β二醇）和睾酮（T）。血清提取物经葡聚糖凝胶LH-20微柱色谱纯化后，分析这些化合物的三氟乙酸衍生物。灵敏度良好：（16.7 pg/ml：A3α二醇，26.7 pg/ml：A3β二醇）。精密度（变异系数=2.75%：A3α二醇，3.11%：A3β二醇）和准确度均优于以往报道。对131名年龄在15-81岁的健康男性和5名年龄在25-60岁的健康女性测定了血清A3α二醇。个体间血清A3α二醇水平存在显著差异，但男性血清（大于20岁）水平与年龄呈显著负相关（r=-0.560，p<0.01）。将这些健康男性分为20-39岁、40-59岁和60-79岁三个年龄组，血清A3α二醇的值（均值±标准差）分别为189.3±77.7（n=20）、127.9±59.5（n=28）和94.9±52.9（n=73）pg/ml。所有年龄组中这种类固醇的水平存在显著差异（p<0.01）。在健康男性（25-77岁，n=77）中，血清A3α二醇与T水平之间存在弱但显著的相关性（r=0.3235，p<0.01）。血清A3α二醇的测定受年龄影响。样本数量对A3α二醇水平均值的判定有很大影响。这些结果表明，在研究这种类固醇时，必须明确这些因素。
