Liposomes as carrier for production of sugar specific antibodies: preparation of antigalactosyl antiserum.

Negatively charged multilamellar liposomes, covalently coupled with p-aminophenylated derivative of D-galactose, have been used to raise galactose specific antibodies in rabbits. The specificity of the antiserum has been characterized by immunodiffusion, quantitative precipitation and hapten inhibition studies. In addition to its specificity against the sugar moiety the antibody population also recognizes the aromatic aglycone portion of the introduced hapten. This offers a new method of raising sugar specific antibodies using liposomes as immunological carriers.