Nerenberg C, Tsina I, Matin S
J Assoc Off Anal Chem. 1982 May;65(3):635-9.
Oxfendazole (methyl 5-(phenylsulfinyl)-2-benzimidazole-carbamate) is a broad spectrum anthelmintic agent designed for use in food-producing animals. A simple radioimmunoassay (RIA) for determination of oxfendazole in plasma was modified for determining oxfendazole in sheep fat. Fat tissue was enzymatically hydrolyzed to an oily residue with collagenase-hyaluronidase, and oxfendazole was then extracted into an acidified aqueous phase. An aliquot of this phase was used directly for RIA. Bound radioactivity was separated from free by using polyethylene glycol-bovine gamma globulin because oils and other components in the aqueous aliquot preclude the use of charcoal for the separation. The lower limit of sensitivity of the assay is 0.003 ppm. Accuracy experiments carried out in the range 0.01-0.5 ppm gave a regression line of y (ng/g) = 0.91 x (ng/g) + 2.89, with r = 0.99. Fat tissue derived from sheep given an oral dose of 6.0 mg/kg was analyzed by this method and by a high pressure liquid chromatographic (HPLC) method. Values obtained by the 2 methods agreed well.
奥芬达唑（5-（苯基亚磺酰基）-2-苯并咪唑氨基甲酸甲酯）是一种设计用于食用动物的广谱驱虫剂。一种用于测定血浆中奥芬达唑的简单放射免疫分析（RIA）方法经改进后用于测定绵羊脂肪中的奥芬达唑。脂肪组织用胶原酶 - 透明质酸酶酶解为油性残渣，然后将奥芬达唑萃取到酸化水相中。该水相的一份等分试样直接用于RIA。由于水相等分试样中的油和其他成分妨碍使用活性炭进行分离，因此使用聚乙二醇 - 牛γ球蛋白将结合放射性与游离放射性分离。该分析方法的灵敏度下限为0.003 ppm。在0.01 - 0.5 ppm范围内进行的准确度实验得到回归方程y（ng/g）= 0.91x（ng/g）+ 2.89，r = 0.99。用该方法和高压液相色谱（HPLC）方法分析了口服剂量为6.0 mg/kg的绵羊的脂肪组织。两种方法得到的值吻合良好。
