Mehta P D, Lassmann H, Wisniewski H M
J Immunol. 1981 Jul;127(1):334-8.
IgG/albumin ratios and presence of oligoclonal bands were studied in neutral pH brain extracts and sera of guinea pigs with chronic relapsing experimental allergic encephalomyelitis (R-EAE). The ratios were found to be significantly increased in central nervous system extracts of animals sacrificed during the first relapse and late stages of the disease relative to those of controls and of animals with acute EAE. These results are consistent with an intracerebral synthesis of IgG found in patients with multiple sclerosis (MS). When the immunofixation (after isoelectric-focusing) patterns of brain extracts and CSF from R-EAE animals were compared with those of controls, R-EAE animals showed distinct oligoclonal IgG bands in the pH region of 7.0 to 9.3, in contrast to diffuse patterns seen in controls. The finding of oligoclonal IgG in brain extracts of these animals further supports the usefulness of R-EAE as an experimental model for MS.
在患有慢性复发性实验性过敏性脑脊髓炎（R-EAE）的豚鼠的中性pH脑提取物和血清中，研究了IgG/白蛋白比率和寡克隆带的存在情况。相对于对照组和患有急性EAE的动物，发现在疾病首次复发和晚期处死的动物的中枢神经系统提取物中，该比率显著升高。这些结果与在多发性硬化症（MS）患者中发现的脑内IgG合成一致。当将R-EAE动物的脑提取物和脑脊液的免疫固定（等电聚焦后）模式与对照组进行比较时，R-EAE动物在pH值7.0至9.3区域显示出明显的寡克隆IgG带，而对照组则呈现弥漫性模式。在这些动物的脑提取物中发现寡克隆IgG进一步支持了R-EAE作为MS实验模型的有效性。
