Mehta P D, Patrick B A, Mehta S P, Wisniewski H M
Immunol Invest. 1985 Aug;14(4):347-54. doi: 10.3109/08820138509022670.
IgG and albumin levels were quantitated in cerebrospinal fluid (CSF) and sera from chronic relapsing (R)-EAE animals using the enzyme linked immunosorbent assay. The animals showed increased CSF IgG compared to that of age-matched animals injected with complete Freund's adjuvant. However, the CSF IgG index suggested no significant increase in IgG synthesis within the central nervous system of R-EAE animals. Matching CSF and sera from R-EAE animals, when compared in immunofixation after isoelectric focusing, showed identical oligoclonal IgG band patterns. Although the clinical and morphologic findings in R-EAE are similar to those seen in multiple sclerosis, the site of IgG synthesis in these two diseases appears to be different.
使用酶联免疫吸附测定法对慢性复发性实验性自身免疫性脑脊髓炎（R-EAE）动物的脑脊液（CSF）和血清中的IgG和白蛋白水平进行定量。与注射完全弗氏佐剂的年龄匹配动物相比，这些动物的脑脊液IgG增加。然而，脑脊液IgG指数表明R-EAE动物中枢神经系统内IgG合成没有显著增加。对R-EAE动物的脑脊液和血清进行等电聚焦后的免疫固定比较时，显示出相同的寡克隆IgG条带模式。尽管R-EAE的临床和形态学发现与多发性硬化症相似，但这两种疾病中IgG合成的部位似乎不同。
