Skare K L, Sietsema W K, DeLuca H F
J Nutr. 1982 Aug;112(8):1626-30. doi: 10.1093/jn/112.8.1626.
The ability of the biliary metabolite retinotaurine to reverse the cornification of vaginal epithelial cells induced by vitamin A deficiency was assessed using a vaginal smear assay. Retinotaurine activity was examined following the intravaginal administration of 10(-12) mol to 10(-8) mol of this compound per vagina. This metabolite exhibited no detectable activity at any dose tested. These results show that retinotaurine cannot be more than 1% as active as all-trans-retinoic acid since retinoic acid shows a response at concentrations of 10(-10) mol per vagina. The low biological activity of this recently identified biliary metabolite suggests that it represents an excretory form of retinoic acid.
采用阴道涂片试验评估了胆汁代谢产物视黄基牛磺酸逆转维生素A缺乏诱导的阴道上皮细胞角化的能力。在每只阴道内给予10⁻¹²摩尔至10⁻⁸摩尔该化合物后，检测视黄基牛磺酸的活性。在任何测试剂量下，该代谢产物均未表现出可检测到的活性。这些结果表明，视黄基牛磺酸的活性不可能超过全反式维甲酸活性的1%，因为维甲酸在每只阴道10⁻¹⁰摩尔的浓度下就会产生反应。这种最近鉴定出的胆汁代谢产物的低生物活性表明它是维甲酸的一种排泄形式。
