Barua A B, Olson J A
Department of Biochemistry and Biophysics, Iowa State University, Ames 50011.
Biochem J. 1989 Oct 15;263(2):403-9. doi: 10.1042/bj2630403.
All-trans-[11-3H]retinoyl beta-glucuronide (RAG) was synthesized in a single step from all-trans-[11-3H]retinoyl fluoride, with a 24% yield. After its intraperitoneal injection into rats, RAG was detected in the blood, liver, intestine and kidney during the following 24 h period. Although the concentration of radiolabelled metabolites decreased with time, RAG predominated at nearly all times in nearly all tissues. Small amounts of retinoic acid (RA) were also universally present, together with unidentified polar metabolites and small amounts of non-polar esters of RA. The major excretion products of RAG in faeces and urine were RA and polar metabolites. Thus RAG, although converted in part to RA in vivo, persists as a major component in blood and tissues for at least 24 h. These observations support the concept that the retinoid beta-glucuronides might serve a physiologically significant role in the function of vitamin A.
全反式-[11-³H]视黄酰β-葡萄糖醛酸苷（RAG）由全反式-[11-³H]视黄酰氟一步合成，产率为24%。将其腹腔注射到大鼠体内后，在随后的24小时内，在血液、肝脏、肠道和肾脏中检测到了RAG。尽管放射性标记代谢物的浓度随时间下降，但在几乎所有组织中，几乎在所有时间RAG都占主导地位。少量的视黄酸（RA）也普遍存在，还有未鉴定的极性代谢物和少量RA的非极性酯。RAG在粪便和尿液中的主要排泄产物是RA和极性代谢物。因此，RAG尽管在体内部分转化为RA，但在血液和组织中至少24小时内一直是主要成分。这些观察结果支持了类视黄醇β-葡萄糖醛酸苷可能在维生素A功能中发挥生理重要作用这一概念。
